Meeting the (N-Terminal) End with Acetylation

(Hyper)tension release by N-terminal acetylation.

A recent study links N-terminal acetylation and N-end rule degradation to blood pressure regulation. N-terminal mutants of Rgs2, a key G-protein regulator, are differentially processed by N-terminal acetyltransferases and the two branches of the N-end rule pathway. This leads to an imbalance in the signaling governing blood pressure.

Identification of N-terminal protein acetylation and arginine methylation of the voltage-gated sodium channel in end-stage heart failure human heart.

The α subunit of the cardiac voltage-gated sodium channel, NaV1.5, provides the rapid sodium inward current that initiates cardiomyocyte action potentials. Here, we analyzed for the first time the post-translational modifications of NaV1.5 purified from end-stage heart failure human cardiac tissue. We identified R526 methylation as the major post-translational modification of any NaV1.5 arginin...

Crosstalk between the Arg/N-end and Ac/N-end rule

Protein degradation in eukaryotic cells is mediated mainly by the ubiquitin (Ub)-proteasome system. One fundamental question in this field is about specific features in a protein that trigger its ubiquitylation and subsequent proteasomal degradation? In other words, the problem of degradation signals (degrons). The first degradation signals to be identified were N-degrons, which comprised desta...

Supporting Information Control of Hsp90 chaperone and its clients by N-terminal acetylation and the N-end rule pathway

Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes

BACKGROUND The N-terminal protein processing mechanism (NPM) including N-terminal Met excision (NME) and N-terminal acetylation (N(α)-acetylation) represents a common protein co-translational process of some eukaryotes. However, this NPM occurred in woody plants yet remains unknown. METHODOLOGY/PRINCIPAL FINDINGS To reveal the NPM in poplar, we investigated the N(α)-acetylation status of popl...